Request for help in releasing Beta-lactamase from the periplasmic space

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Topic: Request for help in releasing Beta-lactamase from the periplasmic space

FS Zollmann
Administrator

posted 10-25-1999 12:24 PM

Dear Sir,

I work on the detection of any plasmids encoding ampicillin resistance in E. coli
and would like to test for beta-lactamase enzyme that is NOT secreted extracellularly.
Are there any methods for releasing this enzyme from the periplasmic space?

Thanks
Sincerely

Kuntaman, MD
Microbiology Department,
Medical Faculty Airlangga University
E-mail: kuntaman@mitra.net.id

IP: 160.45.191.21

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