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 pull down assay
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| Author | Topic: pull down assay |
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seeker unregistered |
posted 02-12-2001 03:02 PM
 
I am having some problems with my pull down assay.I have successfully cloned my bait protein into pCI-NEO vector and prey protein into pCDNA3 vector. I have also sequenced the junctions to ensure they are in frame. I did a co-transfection into cos-1 cells and did a lysis reaction after 12hrs, 24 hrs and 36hrs. When I do a western, I got exactly the same bands for the lanes containing my samples and lanes with the empty vectors alone. I am using anti-myc and anti-flag antibodies. Please give me your precious advice. Thank you IP: 202.156.50.19 |
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