Dye Tagged (DT) Capillary Electrophoresis (CE) (Gel and non-Gel) (G and NG)Info Enterprises Canada

Creating Employment for Economic Growth in the Philippines
Colombie-Britannique (C.-B.) V3B 1G3
Canada
Toll free: +011-236-983-6419

Phone: +011-236-983-6419
Fax: 1-604-941-9022
E-Mail: This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

Description:

We are in the process of consulting for future licensed manufacturers as subsidiaries internationally.

We are gathering business intelligence in the USA for our "Digitel Laser Device" to be developed with Canadian & Philippine manufacturing subsidiaries. A partner in Canada with exclusive rights to use the dye tag is planned and another in the NIR region in the PHILIPPINE ISLANDS.

We will contact candidates to be licensed exclusively with use of our dye-tagged (DT) denaturing electrophoretic protocol using microcapillary non-gel (NG) columns for hi-throughput, hi-speed (capacity) proteomic (and peptide) analysis as well as gel (G) columns for larger samples and more limited runs which beats the competition for fast turn around of our column runs with faster, ease of use not available for conventional dye-tagged gel protocols.

The denatured analytes with attached DT will be isoelectric focused by an electric field gradient along the cartridge of loaded sample with multiple non-gel (NG) capillaries or larger sample gel (G) columns, separation being based on size/MW and net charge for each protein type analyte. 

The feature of running parallel samples (x2), one with denaturing DT and the other with no DT added will allow prep recovery of the sample in question of interest in this type of analysis.

In the long term of interest to our particular independently run Philippine-based subsidiary in the NIR, in the PHIL., is to use our CDMO's prototype "digitel reader" for marketing and capitalization of company facilities with exclusive licensing for the dye tag (DT) reagent product which also will be manufactured multinationally and with licensed established chemical cos. especially in the U.S. of A.  We will possibly approach Sigma Millipore there in future.

The competition we expect or believe are going to field similar analytical machines with laser-driven reading capability without a dye-tag and will rely on less quantitave methods to read analytical peaks and using a universal method of mechanically collecting aliquots that are resolvable by titre and displacement. But without quantitative capability it is less elegant and automated for routine quantitation for the QC runs used in the Food Science & Technology industry protocols for such food-grade analytes creating extra market share for our product.

For both the competition's device readers' non-DT protocols and our own DT protocols, the readers will have to be cost-effective to compete successfully in the very fast-growing and lucrative bioinformatics and metabolomics fields, just beginning for both proteomics and its genomics fields.

There has been mention of prioritizing this type of research for the bioinformatics-led development of the now dubbed direct applied RNA-based regulatory (DARR) protocols led by the invention technologies from the U. of Warsaw, Poland by J. Trylska's and group using the fine biologic PNA-B12 regulator with Vit B12 as the critical carrier through cellular introduction, and in our case a new candidate proprietarily protected here on this website called PNA-K2, with Vit K2 as the carrier, and with linker/spacer as attached to the regulatory PNA moiety of the fine biologic in its macromolecular structure.  The inventor as its author believes that A-prioritization should occur with the development of applications with problems for forage cropping, microbial probiotics, microbial feed additive prebiotics and for large animal metabolism livestock production models, the latter which is believed to be extensively involved with animal production & nutrition, health and animal welfare. 

In the news: [The reader is invited to research the Internet for more of the same news reported here. Including recent reports of what is the Maurice Flex Cartridges(R) by bio-techne(TM)].

Our overall Corporate "game plan" for the Denaturing Gel Tagged Capillary Electrofocused (DGT-CE) technique to displace and compete with the SDS PAGE protocol includes a dual strategy between chemical reagent mfging and digitel machine reader which is laser-based mfging. With a contact in the metro capital area of Manila, the Philippines we reach out with financial services and legal services for Skye Blue Corporation based in Canada. It is understood that a CDMO for reagent contract mfging and CDMO for contract mfging for the biomedical device, the device paralleling bio-techne's(TM) core isoelectric focusing technology with the Maurice Flex cartridges(R) to be set up by our present Principal and Owner first based in Manila and then in Tokyo, Japan; Toronto, Canada; MA USA; Mexico City, Mexico; Abu Dhabi; with licensed machines if agreeable with the likes to Thermo-Fisher (MA, USA), Bio-Rad (USA) and Amersham (AU). As a corp. world-wide we strive for ownership (decision-making) and shareholder control including lifetime ownership of the Detergent-Dye tagging (DT) reagent as licensed to partners through the CEOs partnered with Skye Blue's Corporation or whichever name it "call signs" by outside our other umbrella, in Canada for advertising. The CEO on internal agreement will obtain and underwrite financial loan services and legal services and garner a 40::60 % cut in shareholdings and overall decision-making in day-to-day company affairs. These will be also offered via IPOs to venture capital investors.  Any initial stock offerings will be announced by the CEO and his Board Committee. We are happy at this juncture to announce a Venture Capital fundraising agency in FL USA who can offer a private investor's funds (we are looking at $50M total) perhaps from Canada.

Thermo-Fisher Scientific Ltd.(R) USA could be an interested co-licensee in this new endeavor in electroseparation. Bio-Rad(R) USA is also another potential co-licensee vs their “Substrate-Level” Chromophoric Tagged (“Dye”) Protein PAGE and Mini-Protein Reader in the competition.  We prefer that competition remains active and healthy and that subsuming manouevres be avoided. Note this is also a viable alternative to our method. Amersham(R) Australia was also approached earlier as a first choice for developing the dye-tag reagent. We will try them again in Australia with further negotiations to license through their subsidiarization with growth prospects in Australia.

We have found that our electrophoretic technology will change denaturing SDS-PAGE due to its facility in the laboratory including semi-automated prep work ("twinning"). NB. we are also proposing here isoelectricfocused non-gel (NG) prep separation as another option for use.

Computational analytics will predominate with proteomics with the [LMW]-proteome from secretagogue body fluids or blood plasma, clean fermentation sampling for food-related products, drug discovery with biopharma, endo and neurohormones, enzymes, nutraceuticals, antibodies for immuno analysis and the multiplicity of biomarkered research for metabolomics. Metabolomics is just initiating for agrocropping, livestock production and health for research growth or expansion at this time. 

CDMOs are key to seeing technology succeed into commercialization although we are just initializing venture capital investing with hefty financial fee payments. There is already word on the street in the metro-Manila the Philippines about possible CDMO entities available for long-term contracting by which obtaining services of a research chemist(s) are realized through possible contact with the Dilliman and UPLB campuses and other leading universities there. It should be announced that our own researcher has documented with the science literature one (Pathway B) route or scheme and a second one (Pathway A) remaining undocumented but in theory correct, the latter is to be completed at our CDMO's library when in progress. These are all copyright protected here with all rights reserved and intended for ACS journal publication as things unfold.

The lab bench research chemist will operate documenting the frame work of verifying parameters of the desired endproduct or reagent for its: MW (calculated), reaction to completion via TLC, absorption (lambda max, epsilon), m.p., PMR spectra, IR spectra, LC-mass spectra, and total % yield for the economic parameter to select and approve the preferred commercial route(s). 

___________________________________________________________________________________________________________________________________________ 

FIG. 2  Practical Schema Using Hydrazine (NH2-NH2) Chemistry to Variate the Chemical Structure of Our Molecular Reagent with Only 1 Methylene Group Between the Azo Unsaturation and the Sulfonate Group with a Total of 12-C Length in its Side-chain. 

1. Starting material is Undecanoic Acid.

    CH3(CH)n=9-COOH 

2. Reduction of the Carboxyllic acid to the Aldehyde.

   CH3(CH2)n=9-CHO

3. Alpha-Bromination.

   CH3-(CH2)n=8-CH(Br)-CHO 

4. Adduct formation with the Hydrazide to the Ene-Azo-Amine.

    CH3-(CH2)n=8-CH=CH-N=N-CH2-NH2 

     (Note: Bi-product of the Ene-Amine-Hydrazide.) 

5. Formation of the Ene-Azo-2-Methyl-thio-pyridinium Nitrate via 2-Thiomethylpyrdinium Nitrate.

    CH3-(CH2)n=8-CH=CH-N=N-CH2-2-Methylthiopyridinium(+)NO3(-) 

6. Sulfate Formation with Cl(SO3)H

    CH3(CH2)n=8-CH=CH-N=N-CH2-ONO2 

PRODUCT:  Ene-Azo-Sulfate (Dye-detergent)

     CH3(CH)n=8-CH=CH-N=N-CH2-(SO3)-H  

SKYENEWS: We are making a preliminary announcement of garnering investors for the "surrogate" arm that will exist in the Philippine side of the Pacific Rim (while we also exist at our home base in Poco, British Columbia, on the other) as Skye Blue Association expands its presence on the world-wide web (WWW) in the Vancouver area of Canada who will be drawn from the metro Manila area including CEOs of potential scientific CROs/CDMOs (eventually) who will be asked to convene with expertise and knowledge around our proprietary project of DT CE Proteomics Analyses to contract our the DT fine chemical reagent we wish to have sole proprietary IP ownership from our inventor, D. A. Flores, and exclusive licensing to benefit sister CROs/CDMOs for the Laser-based Reader unit that describes itself as a hi-capacity, hi-speed device using either gel or non-gel (G or NG) microcapillary that can read the proteins and oligopeptides in real time, after being electro-focused in a supposed cartridge. One of the major features of our assemblage is the MW ID/quantification of proteins with standards and unknowns when conducting research analysis of given protein analytes and used to give the dose delivery or titre used in vitro or in vivo. It can also simultaneously detect and collect a "parallel" prep sample for further analyses, re. structural-functional relationships of enzymes, antigenic vaccines, hormonal and various biopharmaceutica for various drug discoveries for R&D. Write in now for more info. We have a proprietary Folio available and descriptive of our invention detailing the synthesis of the fine chemical reagent for CRO work and specs for a competing non-dye-tagged unit. This is desktop published by the Skye Blue Association for all public stakeholders. Join Us! The continuing discussion regards the invention's technical analysis is open to all interested investors. We will convene all our findings and comments in our company website: https://sbinternet.ca . We intend to be world leaders in electrophoresis analysis in future with world-wide marketing and advanced features like AI use and Data Processing Centres (DPC) both from the Philippines and in Canada with data collection.

(c) 2026-2059. D. A. Flores. SKYE BLUE (SB) INTERNET. Port Coquitlam. BC. Canada V3B 1G3          

Last update of this entry: October 01, 2026