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To: Multiple recipients of list HUM-MOLGEN <HUM-MOLGEN@NIC.SURFNET.NL>
Subject: CALL, BIOT: various
From: Arthur Bergen <bergen@AMC.UVA.NL>
Date: Mon, 7 Aug 1995 11:27:50 +0100

This BIOT CALL contains 3 submessages:

1) CALL for DNA banking experience
2) CALL for "promotor finder" experience
3) CALL : RNA binding proteins

The CALL section is open for CALLs for collaboration, OFFERs of
assistance, CALLs for information or other help.
The BIOT section primarily discusses technical problems, companies
and funding and other subjects related to BIOTechnology/Molecular
Biology.
Please REPLY to these CALLs by person to person E-mail, and not to
HUM-MOLGEN, unless your answer is of general interest to the entire
HUM-MOLGEN digital web.

Good CALLs !

Arthur Bergen

************************************************************************

Subj:   CALL- DNA banking

This message was originally  submitted by jeff_kant@PATH1A.MED.UPENN.EDU to the
HUM-MOLGEN list at  NIC.SURFNET.NL.

                       Subject:                               Time:7:21 PM
  OFFICE MEMO          CALL: DNA banking                      Date:8/1/95
For those with banking experience.  A colleague would like to establish a bank
for future DNA extraction from bloods at minimum effort.  He favors either a
commercial kit or sucrose-triton lysis.  What is people's experience with long
term storage of partially prepared DNA, or other recommended suggestions for
such a project.

Jeffrey Kant
University of Pennsylvania
jeff_kant.labmed@path1a.med.upenn.edu
*****************************************************************************

Subj:   call : clontech promotor finder

This  message   was  originally  submitted  by   WMGPJEA@CARDIFF.AC.UK  to  the
HUM-MOLGEN list at  NIC.SURFNET.NL.

I am using the Clontech kit, Human Promotor Finder which is
a vectorette PCR method for amplifying into regions of unknown
sequence.  I have had problems with the protocol and have only been
able to get 1 of the 5 positive controls included in the kit to work.

Has anyone else used this method?
Did you get the controls to amplify?


Dr Philip Asherson
Institute of Medical Genetics
Heath Park Cardiff
CF4 4XN
e-mail: asherson@cardiff.ac.uk

*********************************************************************

Subj:   RNA binding proteins

This message was originally submitted by VOGT@NOVSRV1.PIO1.UNI-HEIDELBERG.DE to
the HUM-MOLGEN  list at NIC.SURFNET.NL.

Dear experts
I'm urgently looking for  conserved sequence boxes of genes coding
for RNA binding proteins. Has anybody suggestions how to screen own
sequence data effiently with all known conserved RNA binding
motifs in the data base.

Thanks a lot for any hint!!!!!!!!!!!!!!!
Peter

*********************************************
Dr. rer. nat. habil. Peter H. Vogt
Section Molecular Human Genetics
Institute Human Genetics & Anthropology
Unuversity Heidelberg
Im Neuenheimer Feld 328
D-69120 Heidelberg
Germany
FAX: (6221) 565332
Phone: (6221) 563924
main interests: @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @
                genetic aspects of spermatogenesis
                human male infertility and ICSI
                Y genes involved in spermatogenesis (AZF loci)
                @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ @


   
 
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