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To: Multiple recipients of list HUM-MOLGEN <HUM-MOLGEN@NIC.SURFNET.NL>
Subject: BIOT: ABI, fluorescent primers
From: Martin Kennedy <MKENNEDY@chmeds.ac.nz>
Date: Thu, 21 Sep 1995 15:49:57 +1200

Date sent:  21-SEP-1995 15:45:17

This message contains three posts relating to ABI fluorescent
genotyping  and primer supply.


Martin Kennedy/Arthur Bergen
BIOT Topic Editors, HUM-MOLGEN

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Date:         Mon, 18 Sep 1995 13:50:13 GMT
From:         Philip J Asherson <WMGPJEA@CARDIFF.AC.UK>
Subject:      Re: BIOT: ABI Genotyping

We have developed our own system for the rapid analysis of ABI
genotype data for linkage studies.  We are currently up to 15 loci per
lane on a 36 well gel, running 3 gels per day.  We have found that
gel to gel variation can produce as much as +/- 2 bp chahnges in
mobility on a 12 cm gel. We have developed our own software to
overcome this.

Philip Asherson
Institute of Medical Genetics

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Date: Mon, 18 Sep 95 14:22:07 EDT
From: Mark.Perlin@J.GP.CS.CMU.EDU
Subject: Re:  BIOT: ABI Genotyping

Our group is developing software that automates the use of ABI (and other
gel-based) genotyping data.  The key innovation is the computer-based
elimination of PCR stutter artifact (1,2) in poly-nucleotide repeat markers.

- Mark
Mark W. Perlin, Ph.D., M.D., Ph.D.
Senior Research Scientist
Computer Science Department
Carnegie Mellon University
Pittsburgh, PA 15213

(412) 521-7421
(412) 521-5204 (FAX)
(1) Perlin, M.W., Burks, M.B., Hoop, R.C., and Hoffman, E.P. (1994). Toward
fully automated genotyping: allele assignment, pedigree construction, phase
determination, and recombination detection in Duchenne muscular dystrophy.
Am. J. Hum. Genet., 55(4): 777-787.

(2) Perlin, M.W., Lancia, G., and Ng, S.-K. (1995). Toward fully automated
genotyping: genotyping microsatellite markers by deconvolution (GMBD). Am.
J. Hum. Genet.: in press.

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Date:         Thu, 21 Sep 1995 09:08:14 +1410
From:         Agnes Tay <mcbtayhn@LEONIS.NUS.SG>
Subject:      fluorescent primers supply?
My collaborators and I are embarking upon a long-term project to study the
genetics of several neurological and neuropsychiatric disorders in
Singapore. We have been promised access to ABI equipment and software which
should greatly speed up everything. Our main concern is the cost of
fluorescent primers and we'd like to know how to get reliable ones at
minimal cost. One of our approaches involves the use of previously defined
microsatellite markers. I've heard that these are sometimes available for
free from some suppliers. Anybody knows how we can apply for these?
Thanks very much,

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