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  Bergen (ioi): BIOT: various; Jan 1996  

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To: Multiple recipients of list HUM-MOLGEN <HUM-MOLGEN@NIC.SURFNET.NL>
Subject: BIOT: various; Jan 1996
From: "Bergen (ioi)" <A.A.Bergen@AMC.UVA.NL>
Date: Wed, 14 Feb 1996 11:11:25 +0100

Note from the editor:

New BIOT messages from Jan 1996!
The BIOT TOPIC is open for requests and offering of protocols, material, 
vectors, clones and biotechnical and molecular inquiries as well as 
sharing of expertise in the molecular genetic and -biological field. 

Also, information about new techniques, methods as well as grants or awards 
for research projects may be placed here. 

Please respond by private E-mail only, unless your response 
is of general interest to the HUM-MOLGEN community.

Arthur Bergen

From:	IN%"twalker@csu.edu.au"  
Subj:	RE: c-myc proteins levels by flow cytometry

This  message was  originally  submitted by  9000114@WIS.RIV.CSU.EDU.AU to  the

Hello. I require your assistance. I wish to quantitate the protein
levels of the oncogene c-myc in tumour tissue from an in vivo rat
model using flow cytometry.

Has anyone had experience or can direct me to a site where I
might ascertian this information.


Todd L. Walker
Charles Sturt University, Australia.
From HSwiger@AOL.COMWed Feb 14 10:13:40 1996
Date: Thu, 28 Dec 1995 19:40:28 -0500
From: HSwiger@AOL.COM
Subject: Intracytoplasmic Sperm Injection

This message was originally submitted by HSwiger@AOL.COM to the HUM-MOLGEN list


For a while I have been attempting to obtain detailed information/current
research on ICSI.  If you have more information on this subject please
contact my e-mail or
phone me at 619-747-4781 or fax at 619-747-0625.  Please send this
information expedititiously.  If you prefer to mail this info:

3913 Summer Way
Escondido, CA  92025

I really appreciate your time and effort in these matters.

Holly S.
From becker@MAILER.UNI-MARBURG.DEWed Feb 14 10:14:48 1996
Date: Wed, 10 Jan 1996 19:26:10 +0100
From: "Becker Stephan Dr." <becker@MAILER.UNI-MARBURG.DE>

This message  was originally  submitted by becker@MAILER.UNI-MARBURG.DE  to the

We are looking for information about the vector pJL3 encoding the
bacteriocin-release-protein. Can somebody tell me the name and address of
the supplier?
Please send your message to Stephan Becker "Becker@Mailer.uni-marburg.de"
Many thanks in advance.


From NSMART@RVC.AC.UKWed Feb 14 10:16:45 1996
Date: Wed, 17 Jan 1996 11:07:01 +0000 (GMT)
From: Nicola Smart <NSMART@RVC.AC.UK>

This message  was originally  submitted by  NSMART@RVC.AC.UK to  the HUM-MOLGEN
list at NIC.SURFNET.NL. 


I have recently started work on down-regulation of PKC in signal
transduction and am intending to introduce random mutations into a
PKC sequence using error-prone PCR. Can anyone advise me on how to
carry out such work, since after extensive searching I have yet to
find anybody using this technique - naturally, all literature describes
working towards error-free PCR!

Any advice will be greatly appreciated.

Royal Veterinary College
Royal College Street
London   NW1 0TU

From selim@CBU.BAYAR.EDU.TRWed Feb 14 10:18:26 1996
Date: Fri, 19 Jan 1996 15:43:19 +0300
From: Selim Uzunoglu <selim@CBU.BAYAR.EDU.TR>
Subject: call: the problem of chromosome number in meiosis

This  message  was  originally   submitted  by  selim@CBU.BAYAR.EDU.TR  to  the

Dear colleaques,

One colleaque, cytogeneticist, said that,  primary spermatoid
starts to divide with 46 chromosomes and end up with 23
chromosomes(haploid) in meiotic division according to his
readings and observations. But from the perspective of molecular
genetics, I believe that it starts to divide after doubling
genetic material(ie;92 chromosome, it becomes first tetraploid).
In the end of meiosis, four haploid sperm or one oocyte form. He
says that he could not get any experimental photo showing 92
chromosome(tetraploid) containing cell stage in chromosome
I told him, molecular geneticist and cytogeneticist are studying
different levels of genetic material and therefore their
concepts and language are not the same often.
These are my questions:
1- How can we match this two level information in coherent way?
How much valid this cytogenetic information in clinical cases
without contradiction with molecular genetic data?
2- Are there any information or publication containing
experimental photos related to chromosomal number of each stage
of meiotic division in human spermatogenesis or oogenesis?
3- As a molecular geneticist, is it still reliable to use
concepts such as chromosome, chromatid,chromatin ,which their
meaning have historical dimension.
 Thank you very much for all contribution made in advance
email: selim@cbu.bayar.edu.tr

From aianhes@IMAGEM.IBILI.UC.PTWed Feb 14 10:19:37 1996
Date: Mon, 29 Jan 1996 13:23:58 +0100
From: Ana Margarida Ianhes <aianhes@IMAGEM.IBILI.UC.PT>
Subject: Restriction map analysis

This  message was  originally  submitted by  aianhes@IMAGEM.IBILI.UC.PT to  the

I'm looking for a program that allows Restriction map analysis. I wish to
know where I can find it.
        Thanks for your attention

                Ana Margarida Ianhes
From Gary.Franklin@DEVBIOL.UU.SEWed Feb 14 10:20:23 1996
Date: Mon, 29 Jan 1996 18:36:41 +0100

This  message was  originally submitted  by Gary.Franklin@DEVBIOL.UU.SE  to the

To all fellow HUM-MOLGEN subscribers:

Hello ! I am currently in need of two things for a coming project and would
be grateful if anyone "out there" could provide me either with the
materials themeselves, or suggest a possible source which I could contact.

The first thing is a CAT reporter plasmid containing the HSV TK promoter
(preferably with lots of nice cloning sites); I haven't managed to find a
commercially available construct sold by any of the "usual" sources :-
maybe you know of one ?

The second thing I would be extremely interested in would be a plasmid for
use in transfection assays containing two different reporter genes (say CAT
and ▀Gal) and intervening cloning sites: if such a plasmid had the two
reporter genes arranged in opposite orientations, so much the better.

If anyone has any suggestions, I would be very happy to hear from you !

Gary Franklin


From e013mr02@AREA.BA.CNR.ITWed Feb 14 10:20:51 1996
Date: Wed, 31 Jan 1996 18:13:11 +0100
From: Mariano ROCCHI <e013mr02@AREA.BA.CNR.IT>

This  message  was  originally  submitted  by  e013mr02@AREA.BA.CNR.IT  to  the

I am looking for a cosmid library specific for chromosome 2. Grateful for
any indication.
Mariano Rocchi

Dr. Mariano Rocchi
Istituto di Genetica
Via Amendola 165/A
70126 Bari - Italy
tl  +39-80-544.3371
fax +39-80-544.3386
e-mail m.rocchi@area.ba.cnr.it

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