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  Bergen (ioi): BIOT, various, Feb 1996  

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To: Multiple recipients of list HUM-MOLGEN <HUM-MOLGEN@NIC.SURFNET.NL>
Subject: BIOT, various, Feb 1996
From: "Bergen (ioi)" <A.A.Bergen@AMC.UVA.NL>
Date: Fri, 8 Mar 1996 11:31:59 +0100

Note from the editor:

With a single E-mail you can now reach approximately 3500 colleauges or
interested parties in genetics and molecular biology from approximately 60
countries world-wide, for free!

The BIOT section is open for messages concerning biotechnology and
molecular genetics and molecular biology. These messages include
sharing of experimental expertise, requests for vectors, clones, cel lines
etc., grant info and requests, info about new techniques, protocols
diagnostic methods, etc.
Companies can post messages in this section, too, but only if these messages
are clearly of (general) interest to HUM-MOLGEN subscribers.
Please send high-quality messages only.

Please respond to BIOT messages by private E-mail, unless your reply is
of general interest to the entire HUM-MOLGEN community.
Some replies may be at the end of this file.

Arthur Bergen


Date: Mon, 05 Feb 1996 21:39:06 -0500
From: Barry Mellovitz <yisber9@EARTHLINK.NET>
Reply to: Human Molecular Genetics Editors <ED-MOLGEN@nic.SURFnet.nl>

This  message   was  originally  submitted  by   yisber9@EARTHLINK.NET  to  the


I'm looking for protocols on mouse for looking at meg. growth or platelet
increases, etc. It seems that it's not so simple to find just one assay
in-vitro that will look for something that is beneficial for
thrombocytopenia or even just an increase in megs. I've had the same cmpd
give me many cfu-meg alone, while with IL-3 it only increased the amount of
single megs in mixed colonies. I've never tried looking at dosed animals,
since that precludes a large volume of cmpds to screen in a short time. But
I also need to find these protocols, which I will anyway, but would like to
get any help from anyone experienced in theese procedures. I've used
acetylcholamine for small progenitor megs,(and know that some use it for
LTC assays for amount of megs) but haven't looked at platelet numbers or
size, as well as meg size. I have access to a facs, and determining numbers
wouldn't be a problem, but would like to discuss more practical issues,
such as which cytokines to use. I've tried a few and had very good success,
but like I said, didn't look at the volume of the nucleus for the 'n'
number or the amount of platelets.  I'm unfamiliar with aggregation
techniques or their purpose, and the issue of AFP which I believe is an
autoimmune destruction of your own platelets, is complicated since the
person has an excess of megs, but a great lacking of platelets.
Thanks for any input,

I'm also on compuserve -

Barry Mellovitz
Abbott Laboratories
Dept of Growth Factors

>From roizes@INFOBIOGEN.FR
From: Gerard Roizes <roizes@INFOBIOGEN.FR>

This message was originally submitted by roizes@INFOBIOGEN.FR to the HUM-MOLGEN
                                                February 6th 1996

        I have received the information concerning the new genetic
diagnostic technology developped by Integrated Genetics, called MASDA.
        I wish to get into contact with Integrated Genetics, but have no
address available, nor Telephone number or email.
        I would be gratefull to you if you could provide me with this
        Thank you very much in advance.
                                        yours sincerely,
                                                Gerard Roizes

Institut de Biologie
4 Bvd Henri IV
34060 Montpellier
Phone: (33) 67 60 11 23
Fax:   (33) 67 60 94 78
email: lovelace.infobiogen.fr

>From agoffinet@QUICK.CC.FUNDP.AC.BE
From: andre goffinet <agoffinet@QUICK.CC.FUNDP.AC.BE>
Subject: in situ PCR

This message was originally  submitted by agoffinet@QUICK.CC.FUNDP.AC.BE to the

Dear Editor:

I wonder whether anyone connected might have experience with
using in situ PCR to reveal rare messages on frozen sections.
We have currently difficulties setting up this technique and
would like to spend some time abroad (in Europe) to learn the
necessary skills.

Dept Physiologie Humaine
FUNDP Med. School
61, rue de Bruxelles
B5000 Namur, Belgium

Tel: 32 - 81 - 724277
Fax: 32 - 81 - 724280

>From hsibul@EBC.EEFri Mar  8 10:31:23 1996
Date: Wed, 14 Feb 1996 16:19:57 +0200
From: Hiljar Sibul <hsibul@EBC.EE>

This message was  originally submitted by hsibul@EBC.EE to  the HUM-MOLGEN list

Dear Editor,
[ I hope this is the right place to get the message to the net

I am interested in the cell line that would lack functional
low-density lipoprotein receptors ( LDLR ).
This would probably be derived from a patient with homozygous form of
familial hypercholesterolemia ( FH ).
If anyone has such a cell line or information , where it could be
obtained from, please reply directly by email.
I would also appreciate the complete sequence (or restriction map) of
a plasmid designated pLDLR 12 or 22. Unfortunately it was not to be
found in currently available vector databases.

Hiljar Sibul
Estonian Biocentre


Hiljar Sibul
Estonian Biocentre               e-mail: hsibul@ebc.ee
University of Tartu              phone:  372-7-420210
23 Riia St.
2400 Tartu
Estonia, Europe                  fax:    372-7-420286


Date: Wed, 14 Feb 1996 13:18:49 -0500 (EST)
From: Eva Czarnecka-Verner <evaczar@MICRO.IFAS.UFL.EDU>
Reply to: anair@gnv.ifas.ufl.edu

Hello to everybody:

I am very interested in obtaining plasmids containing coding sequences for
the full length LexA DNA binding domain, as well as, LexA DNA binding site
present in multiple copies.  Please, let me know if there is someone out
there who can help.  Thank you.
Eva Verner

Microbiology Department
University of Florida
Bldg. 981, P.O. Box 110700
Gainesville, Fl 32611-0700

>From scott_jokerst@DATA-TRANSPORT.COM
Subject: Re: Restriction map analysis

This message  was originally  submitted by  scott_jokerst@DATA-TRANSPORT.COM to


I went to Biological Data Transport's web site at:


and queried for restriction mapping.  (You can query for many life science
related topics, and the site is becoming more comprehensive daily).

Two companies come up out the system with that query, the first one being
DNASTAR, Inc., which is offering the LASERGENE software for evaluation.  A
click on that link will bring you directly to DNASTAR's LASERGENE product

Hope that helps,


At 4:23 AM 1/29/96, Ana Margarida Ianhes wrote:
>This  message was  originally  submitted by  aianhes@IMAGEM.IBILI.UC.PT to  the
>I'm looking for a program that allows Restriction map analysis. I wish to
>know where I can find it.
>        Thanks for your attention
>                Ana Margarida Ianhes

  ---> R. Scott Jokerst            scott_jokerst@data-transport.com  --->
--->   Biological Data Transport   http://www.data-transport.com       --->
 --->  510-648-8229                510-648-8279 (FAX)               --->

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