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  Bergen (ioi): BIOT: various dec 1996  

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To: Multiple recipients of list HUM-MOLGEN <HUM-MOLGEN@NIC.SURFNET.NL>
Subject: BIOT: various dec 1996
From: "Bergen (ioi)" <A.A.BERGEN@AMC.UVA.NL>
Date: Mon, 13 Jan 1997 10:29:20 +0100

New BIOTechnology and molecular biology related messages.

Please send high quality messages only related to the correct TOPIC.
Other messages may be refused or get lost without further notification.
Please respons by private E-mail only.

Happy new year!
Arthur Bergen


Subject: (Fwd) HLA-Typing

Thomas.Dick@Ac.cybercity.de (Thomas Dick) sent the following comments:

we want to investigate the genomic sequence of the HLA-DRB1-
gene of some of our patients to elucidate the HLA
association of autoantibodies occuring in these patients.
For this purpose we need the genomic intron-sequence of the
HLA-DRB1-gene especially in front of the secound exon to
construct a primer to start using patients DNA as template.
Can anybody tell us were we can find the sequence data ?
Thank you in advance

Dr. Thomas Dick, Rheumafroschungsinstitut Aachen, Germany

E-Mail.:          Thomas.DICK@AC.CyberCity.de
Voice.:           Germany +49  241 /6096-4061 or. 4060
Fax.:             Germany +49  241 /6096-1964
Paper-Mail.: Dr.  Thomas Dick, Rheumaforschungsinstitut
                  Haupstr.21, D-52066 Aachen, Germany

Server protocol: HTTP/1.0
Remote host: susi.Informatik.RWTH-Aachen.DE
Remote IP address:

Subject: (Fwd) Protein sequencing

From:          =?iso-8859-1?Q?Ren=E9?= Zimmermann <rzimmer@KERCKHOFF.MPG.DE>
Subject:       Protein sequencing

This  message  was  originally  submitted by  rzimmer@KERCKHOFF.MPG.DE  to  the

Dear colleagues,
does anybody know a company doing commercial protein sequencing ?
Any information is appreciated.

R. Zimmermann

Dr. Rene Zimmermann
Molecular Cardiology Laboratory
Dept. of Experimental Cardiology
Benekestrasse 2
D-61231 Bad Nauheim
Tel.: (0)6032/705-404
Fax:  (0)6032/705-419
e-mail: rzimmer@kerckhoff.mpg.de

From: Alex Dobrovic <adobrovic@MEDICINE.ADELAIDE.EDU.AU>

This message was originally  submitted by adobrovic@MEDICINE.ADELAIDE.EDU.AU to

We are looking for a protocol using TdT  and P-33 dATP to label antisense
oligonucleotides for in situ hybridisation of tissue sections.Any comments
onthe length of tail that's desirable, the need to use cold nucleotides etc
would be appreciated.

Alexander Dobrovic, Ph. D.
Chief Scientist
Department of Haematology-Oncology
The Queen Elizabeth Hospital
Woodville, SA 5011, Australia

Affiliate Senior Lecturer
Department of Medicine
University of Adelaide

Tel 61-8-8222 6884
Fax 61-8-8222 6046 (note new numbers as of August 1996)


From: Amin El-Nashar <ask@INTOUCH.COM>
Reply to: Human Molecular Genetics Editors <ED-MOLGEN@NIC.SURFNET.NL>

This message was originally submitted by ask@INTOUCH.COM to the HUM-MOLGEN list

plase informate us about if you have a researsh about relation between
oncogenes (myc family ) and brain tumours
fax :3612221
email : ask@intouch.com


>From dfarkas@beaumont.eduMon Jan 13 10:13:18 1997
From: "Daniel H. Farkas, PhD" <dfarkas@beaumont.edu>
To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@NIC.SURFNET.NL>
Subject: Trisomy 18, trisomy 13

This message was originally submitted by dfarkas@BEAUMONT.EDU to the HUM-MOLGEN

Last week, a child was born and lived for about an hour.  There were
several, severe anomalies but no diagnosis was made.  Before the
autopsy, liver and spleen specimens were sent to my lab for DNA
Extraction and Banking (at the request and with permission of the
parents).  The liver DNA was badly degraded but the spleen
DNA is excellent and we have over 600 micrograms of it.  Cytogenetics
was also requested but no cells grew.  The attending suspects Trisomy 18
or 13 and would like to have a diagnosis in this case.  Now that
cytogenetics is not possible, does anyone out there know of a lab that
can do trisomy analysis on a DNA specimen?  Seems like it would take
some pretty finely tuned quantitation with chromosome specific probes,
perhaps by Southern blotting with an internal control.  Anyone's help
would be greatly appreciated.  Thanks.

Happy Holidays,

Dan Farkas, PhD
Co-director, Molecular Probe Lab
Wm. Beaumont Hosp.
(810) 551-5077-phone
(810) 551-3694 (fax)

From: Joe Lorenz <jlorenz@geneticid.com>
Subject: CALL: nonisotopic sequencing

This  message   was  originally  submitted  by   jlorenz@GENETICID.COM  to  the

Dear colleagues:

I am presently in a lab that does not utilize radioisotopes.  I have
been trying to sequence mtDNA PCR product using cycle sequencing and
silver staining with very limited success.  I was wondering whether
there is anyone out there who could either help me refine my technique
or advise me about other non-isotopic squencing systems.  Thanks in

Joseph G. Lorenz, Ph.D.
Analytical Genetic Testing Center
7808 Cherry Creek S. Dr., #201
Denver, CO 80231
(303) 750-2023
(303) 750-2171 fax

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