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Subject: BIOT: June 1997 various
From: "Bergen (ioi)" <A.A.BERGEN@AMC.UVA.NL>
Date: Thu, 19 Jun 1997 10:20:26 +0200

New BIOTs!

The BIOT section is open for requests and offers of information regarding
biotechnology, molecular biology and molecular genetics

You can reach over 4250 of your colleagues, and on average you may expect
up to twenty replies to a single message. This service is absolutely FREE
of charge. Help yourself by helping your colleagues!

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 interest to the entire HUM-MOLGEN community

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Edward Wilcox
Arthur Bergen
(CALL editors)


1) Request for E. coli strains
2) Request for an expert in RNA splicing
3) Request for help in multiplexing on a 377 ABI with Genescan software
I would like to be working on the transfer of ampicillin resistance
plasmids by Mating and conjugation methods.

I need: Escherichia coli strain:
         a. Ampicillin sensitive (MIC Less than 4-8 Microgram/ml)
         b. F - (negative)
         c. Optionally: Lac - (negative)
            Nalidixic or/and Streptomycin Resistant
Maybe strain CSH or something similar
I have contacted: J H Miller (UCLA, USA) but with no response (yet)


Thanks and I hope to hear from you soon.

Best regards,

Dr. Kuntaman
Microbiology Department, Medical Faculty Airlangga University
Jl. Prof. Dr. Moestopo 47 Surabaya, Indonesia
Fax: 062 031 522472
E-mail:  "dr. Indrayana" <indranet@INDO.NET.ID>
Dear colleagues
Are you expert in RNA splicing?!

   In the course of mutation analysis on one of the mismatch repair genes,
several unusual short transcripts were observed. Basically, RNA has been
extracted from peripheral blood followed by RT-PCR. In some patients,
shorter products were observed. On sequencing the short transcripts, it was
found that the middle of one exon joins to the middle of another exon and
the exons in between are missing. No alteration was found at splice
junction sites or within the corresponding exons. One of these aberrant
transcripts was found in four unrelated individuals. Another two mRNA
deletions exhibit AAAGA before and after the breakage points.

What is the possible explanation for these mRNA deletions?

Any comments would be greatly appreciated.

A. Davoodi
E-mail: 9315831d@udcf.gla.ac.uk
Dear Colleagues,
   Currently we are using the ABI377 and Genescan software for genotyping.
We would like to develop a robust multiplex PCR for large scale projects.
Does anybody have experience with multiplexing using the PTC100 by MJ
Research ? All replies will be posted.

Thank you.
Min Ae Lee, M.D.
Cardiovascular Division
Brigham and Women's Hospital
phone: 617-732 8174
fax:   617-264 6830
E-mail: mal@calvin.bwh.harvard.edu

Edward Wilcox
Laboratory of Molecular Genetics
National Institute on Deafness and Other Communication Disorders
5 Research Court, 2A-19
Rockville, MD 20850-3227  USA
Tel (301) 402-4162
FAX (301) 480-8019
E-mail  edwilcox@pop.nidcd.nih.gov
Dr. Arthur A.B. Bergen
Department of Ophthalmogenetics
The Netherlands Ophthalmic Research Institute (IOI)
Royal Academy of Sciences of the Netherlands (KNAW)

** Snail-mail: **           ** FAX: **             ** E-mail: **

P.O.Box 12141               (+31)206916521         A.Bergen@IOI.KNAW.NL
1100 AC  Amsterdam
The Netherlands

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