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Subject: BIOT: for November
From: Arthur Bergen <a.bergen@ioi.knaw.nl>
Date: Mon, 21 Dec 1998 09:21:04 MET
Organization: ioi.knaw.nl
Priority: normal

New BIOTs!

The BIOT section is open for requests and offers of information
regarding molecular biology and molecular genetics (protocols,
techniques, products, collaboration, etc.).

You can reach over 5016 of your colleagues, and on average you may
expect up to twenty replies to a single message. This service is
absolutely FREE of charge. Help yourself by helping your colleagues!

- Please send high quality messages only, including full name,
address and purpose.
- Please use the appropriate TOPIC subject heading in your message.
- Please state non-trivial questions only.
- Please reply by private E-mail only, unless your request is of
general interest to the entire HUM-MOLGEN community

Messages may be refused without further notification.

Edward Wilcox
Trevor M. D'Souza
(BIOT editors)

This BIOT message contains:

1) Need help on isolation of genomic DNA from slides
2) A request for P19 cell line
3) I need information on competitive PCR
4) Need information about Bio-Informatics
5) Need information regarding genetic studies on Chronic Idiopathic
Thrombocytopnenic purpura
6) Where can I find information on the gene/s that contribute to eye color?

I am trying to extract genomic DNA from lung tissues that were fixed
on slides and am having difficulty recovering DNA that can serve as
template for PCR amplification.  These slides were stained with H&E
and were paraffin embedded.  Does anyone have experience
isolating human genomic DNA from tissues fixed on slides and
successfully amplifying it by PCR? Thank you for your help.

Nancy C. Arbour, Ph.D.
Assistant Research Scientist
Department of Internal Medicine
University of Iowa
2182 Med Labs
Iowa City, IA 52242
E-mail: Nancy-Arbour@MAIL.INT-MED.UIOWA.EDU

I am a graduate student at the University of Cincinnati and have
been having problems with the P19 mouse EC cells that we have been
getting from ATCC. The company insists it is not their cell line
that is contaminated, but our samples (all from the same lot) seem to
be contaminated upon arrival. Does anyone else have this problem?
Does anyone have viable P19 cells that we could obtain?  We have
already lost several weeks to this problem.  Any help would be
greatly appreciated.

Alex Ramirez
Dept. of Biological Sciences
University of Cincinnati
E-mail: ramireae@email.uc.edu

I am interested in competitive PCR techniques using a plasmid as
control substrate for amplification. I have never used such methods
and am looking for troubleshooting advice. I am also
looking for new methods for quantitative mRNA and cDNA analysis
based on PCR.

Wojciech Mlynarski, MD, MSc
Molecular Biology Unit,
Institute of Peadiatrics,
Medical University of Lodz
Sporna Str 36/50, 91-738 Lodz
phone/fax +48 42 6562902
E-mail: mlynek@alef.am.lodz.pl

How do I locate Bio-Informatics professors and their students? Is
there some sort of Bio-Informatics journal? Please advise.

Shellie B. Williams
E-mail: williams@BIOWEBDIR.COM

I am a scientific doctor at the Research Institute of Pediatrics in
Moscow, Hematology department. My recent theme of studies is Chronic
Idiopathic Thrombocytopnenic purpura. I wonder if anyone can provide
me with information regarding possible genetic research done in
this field, or give me an idea of specific genetic studies I can
do. Any advice is highly appreciated.

Dr. Victoria Khouloudi,
Research Institute of Pediatrics
Lomonossovsky pr. 6/2,
Tel # 7 095 - 134 15 82
Fax # 7 095 - 280 25 12
E-mail: VKhouloudi@intsos.com

My AP Biology class and I are trying to find information on the
gene/s that contribute to eye color.  We understand brown and blue,
but the other colors confuse us.  We would greatly appreciate the
help of anyone who could give us information.  Thank you for your

Emily Dean

E-mail: naedylime@hotmail.com
Dr. Arthur A.B. Bergen
Department of Ophthalmogenetics
The Netherlands Ophthalmic Research Institute (IOI)
Royal Academy of Sciences of the Netherlands (KNAW)

** Snail-mail: **           ** FAX: **             ** E-mail: **

P.O.Box 12141               (+31)206916521         A.Bergen@IOI.KNAW.NL
1100 AC  Amsterdam
The Netherlands

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