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Arthur Bergen: BIOT: for August | ||||||||||||||||
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To: HUM-MOLGEN@NIC.SURFNET.NL Subject: BIOT: for August From: Arthur Bergen <a.bergen@ioi.knaw.nl> Date: Mon, 14 Sep 1998 14:49:20 MET Organization: ioi.knaw.nl Priority: normal New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 5016 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues ! - Please send high quality messages only, including full name, address and purpose. - Please use the appropriate TOPIC subject heading in your message. - Please state non-trivial questions only. - Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community Messages may be refused without further notification. Edward Wilcox Trevor M. D'Souza (BIOT editors) ------------------------------------------------------------------- This BIOT message contains: 1) Is there a better method than alkaline phosphatase direct labeling with chemiluminescence detection for DNA probes? 2) How does one evaluate if overloading the oocyte protein synthesis system upon injection of cRNA has occurred? 3) A search for a lysogenic strain of E. coli whose lytic genes are under the control of a temperature-sensitive (or other inducible) promoter/suppressor and which can be selected against using an antibiotic resistance gene. 4) Will someone provide references regarding the genes for long QT syndrome? 5) A desire to obtain an actin promoted neomycin gene. ****************************************************************************** Dear Sir/Madam: Is there anyone with experience in using alkaline phosphatase direct labeling and chemiluminescence detection? I would like to use Alkaline Phosphatase labeling on an oligonucleotide probe (~ 30 mers). There are two kits we are considering (Amersham and Boehringer). Any suggestions would be appreciated. Sincerely, Pornprot Limprsert M.D., Ph.D. Human Genetics Unit Department of Pathology Faculty of Medicine Prince of Songkla University Had Yai, Songkhla 90110 Thailand Tel: 66-074-212070 to 9 ext 1584 Fax: 66-074-212908, 212903 ****************************************************************************** Dear Colleagues, I am doing some experiments in which proteins are expressed by injecting cRNA (for potassium channels) into Xenopus oocytes. From the whole cell current I can roughly calculate how many channels are on the surface membrane--which turns out to be a lot. I want to figure out if I am overloading the oocyte protein synthesis system. How should I evaluate this? Two figures would help me. First, what is turnover rate of, say, ion channels in oocyte. Second, what is the surface membrane protein density. Any suggestions will be very much appreciated. Zheng Jie jie.zheng@yale.edu ****************************************************************************** Dear colleagues, I am currently looking for someone who could supply me with a lysogenic (lambda?) phage strain whose lytic genes are under the control of a temperature-sensitive (or other inducible) promoter/suppressor and which can be (ideally) selected against a resistance gene. Of course an E. coli lysogen with the same properties as above would work as well. We would be very glad if someone could give us such a strain. Thank you very much in advance for your help, regards, Stefan Schmidt Schmidt Stefan <Stefan.Schmidt@GPC-AG.COM> ****************************************************************************** Dear Colleagues, I have several patients with long QT syndrome and family DNA samples. What we want to do now is analyze these samples with specific PCR primers to identify any recognizable mutations or alterations. It would be very helpful if someone can provide the information or references for the DNA or mRNA sequence for the genes of long QT syndrome so that we can design several primers. Thanks in advance. Min Yoo Department of Biology Keimyung University Taegu, 704-701 Korea Tel and Fax) 82-53-580-5537 E-mail) ymin@kmucc.keimyung.ac.kr Min Yoo, Ph.D. ymin@kmucc.keimyung.ac.kr ****************************************************************************** Dear Colleagues, Does anyone have a neomycin gene under the actin promoter that they are willing to share with me. Lisa Shang <shangl@MUSC.EDU> Remote IP address: 128.23.125.145 ****************************************************************************** ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************
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