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Subject: BIOT for January
From: Arthur Bergen <a.bergen@ioi.knaw.nl>
Date: Wed, 17 Feb 1999 07:36:35 MET
In-Reply-To: <v03102800b2ef360a5889@[]>
Organization: ioi.knaw.nl
Priority: normal

Date:          Tue, 16 Feb 1999 09:57:40 -0500
Reply-to:      Human Molecular Genetics Editors <ED-MOLGEN@NIC.SURFNET.NL>
From:          Edward Wilcox <wilcoxe@NIDCD.NIH.GOV>
Subject:       BIOT for January

New BIOTs!

The BIOT section is open for requests and offers of information regarding
molecular biology and molecular genetics (protocols, techniques, products,
collaboration, etc.).

You can reach over 5500 of your colleagues, and on average you may expect up
to twenty replies to a single message. This service is absolutely FREE of

- Please send high quality messages only, including full name, address and
- Please use the appropriate TOPIC subject heading in your message.
- Please state non-trivial questions only.
- Please reply by private E-mail only, unless your request is of general
  interest to the entire HUM-MOLGEN community.
- Messages may be refused without further notification.

Edward Wilcox
Trevor M. D'Souza

(BIOT editors

This BIOT message contains:

[1] I need useful resources for genetics project.
[2] Free Online PCR Resources.
[3] Does someone have a protocol for extraction of DNA from human skeletal
[4] I need information about interaction between cationic polymers and DNA
    or RNA.
[5] I need information about mutations/polymorphisms in human C-reactive
[6] I would like information on tissue array technology.



My niece is doing a genetics project and is looking for useful resources -
posters, handouts, general information, as it is a science fair project.


Patricia Rockman

E-mail: patrock@GLOBALSERVE.NET

TOPIC: FREE Online PCR Resources

The Alkami Biosystems web site http://www.alkami.com is devoted to offering
FREE polymerase chain reaction (PCR) resources and information.

Dot blots and reverse dot blots are techniques where PCR products are
hybridized with allele specific oligonucleotides (ASOs) for the purpose of
scanning for mutations, detecting RNA, cancer, or pathogens.

Alkami Biosystems also offers you resources for primer design, PCR methods,
troubleshooting, polymerases, and other PCR related topics. The site is
constantly updated in response to our visitors' requests.

"Simplified Solutions for Complex Questions"
P.O. Box 11216, Berkeley, CA 94712 USA.

E-mail:  stewartl@ALKAMI.COM

I would like to know if someone has a protocol for Extraction of DNA from
human skeletal remains.


Dr. Victor Pardini - Brazil

E-mail: vpardini@labhpardini.com.br

Hello, I need information about interaction between cationic polymers and DNA
or RNA. Does someone know where such information can be found? Thanks in

Ariel Casanova

E-mail:  aec@INAME.COM

I am interested in information -- anecdotal or otherwise-- about
mutations/polymorphisms in human C-reactive Protein and any associated
clinical manifestations.

Dr. Eluem R. Blyden
Genetic Designs

E-mail:  geneticdesigns@MINDSPRING.COM

Tissue array technology

I would like information on tissue array technology - a new method for looking
at multiple tissue sections on a single slide after transferring multiple plugs
to a single paraffin block. I would like to purchase any of the tools and
block formers that have been developed to make this practice efficient. I
also appreciate a list of potential problems when using this technique.

Dr. Raymond Clarke
Cancer Care Centre
St. George Hospital
NSW 2217

E-mail:  r.clarke@unsw.edu.au
Dr. Arthur A.B. Bergen
Department of Ophthalmogenetics
The Netherlands Ophthalmic Research Institute (IOI)
Royal Academy of Sciences of the Netherlands (KNAW)

** Snail-mail: **           ** FAX: **             ** E-mail: **

P.O.Box 12141               (+31)206916521         A.Bergen@IOI.KNAW.NL
1100 AC  Amsterdam
The Netherlands

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