CLONING AND ANALYSIS OF LARGE DNA MOLECULES
April 10 - 23, 1996
Invited Speakers: Lectures by outside speakers on topics of current interest will supplement the laboratory work.
Program:
This course will cover the theory and practice of manipulating and cloning high molecular weight DNA. The course will focus on the use of yeast artificial chromosome (YAC). bacterial artificial chromosome (BAC) and bacteriophage P1 cloning systems for library construction and techniques of pulsed field gel electrophoresis (PFGE). Lectures and laboratory work will include an introduction to yeast genetics, the isolation and manipulation of high molecular weight DNA from a variety of sources, and preparative and analytical PFGE. Clones will be produced and characterized by several approaches including library screening, contig assembly, long range restriction and RecA-assisted Restriction Endonuclease (RARE) cleavage mapping, and recovery of YAC ends.
Registration :
Information on how to register is available online.
Deadline for Abstracts: Application Deadline is January 15, 1996
Email for Requests and Registration: meetings@cshl.org