Program:
In this workshop, recombinant human CAII from a bacterial expression system is used to provide
participants with a model for learning the principles of:
Protein Isolation and Purification
Tris-Glycine and Tris-Tricine SDS-PAGE Gels
IEF and Two-Dimensional Gel Electrophoresis
In-gel Proteolytic Digestion for Peptide Mapping and Microsequencing
Enzyme Activity and Protein Concentration Assays
Immunoblotting
Students spend approximately 75% of their time at the bench performing experiments that have
been designed using a problem-solving approach. Lab lectures by UF faculty and ICBR personnel
emphasize practical applications of protein characterization technology and ground-breaking uses
of advanced methods such as NMR, capillary electrophoresis and mass spectrometry.
Registration :
http://ed2.biotech.ufl.edu/ICBR-EDUCORE/info/courses/reg_form.html
Deadline for Abstracts: None
Email for Requests and Registration: wmsetliff@biotech.ufl.edu