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Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
April 16 - 26, 2004
The frog Xenopus is an important vertebrate model for studies of maternal factors, regulation
and molecular mechanisms of tissue inductions and regulation of cell fate decisions. In addition,
Xenopus oocytes and embryos provide a powerful system in which to conduct a number of cell
biological and gene regulation assays. This course will provide extensive laboratory exposure to
the biology, manipulation and use of oocytes and embryos of Xenopus. The course consists of
intensive laboratory sessions, supplemented by daily lectures and demonstrations from experts in
cellular, experimental and molecular development. Areas to be covered include: (i) care of adults;
(ii) oocyte isolation and embryo production; (iii) stages of embryonic development and anatomy;
(iv) whole mount in situ hybridization and immunocytochemistry; (v) microinjection
of eggs and oocytes with lineage tracers, DNA constructs, mRNA and antisense oligonucleotides;
(vi) micromanipulation of embryos, including explant and transplantation assays; (vii) in vivo time
lapse confocal imaging; (viii) preparation of transgenic embryos; and (ix) use of Xenopus
tropicalis for genetic analyses. This course is suited both for investigators who have had no
experience with Xenopus, as well as those who have worked with Xenopus and wish to
learn new and cutting edge techniques. All applicants should have current training in molecular
biology and some knowledge of developmental biology.This course is supported with funds provided by the National Institute of Child Health and
Human Development, the National Science Foundation and the Howard Hughes Medical Institute .
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Organized by:
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Ken Cho & Jan Christian |
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Invited Speakers:
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(Speakers in the 2003 course included:)
Raymond Keller, University of Virginia
Mary Lou King, University of Miami School of Medicine
Kristen Kroll, Washington University School of Medicine
Malcolm Whitman, Harvard Medical School
Aaron Zorn, Children's Hospital Medical Center
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Deadline for Abstracts:
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Application deadline: January 15, 2004
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Registration:
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Please apply here
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E-mail:
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meetings@cshl.edu
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