Chiral Pharmaceuticals: Analysis & Separation

This two-day course is designed for chemists and research scientists in the pharmaceutical industry whose job functions involve analysis and separation of enantiomers, determination of racemic mixture and the economics and applications of optically active chiral pharmaceuticals. No expertise in chiral chemistry is necessary. By the end of this course, attendees will understand methods commonly employed to analyze and to separate chiral molecules.

Learning Objectives

Physical and chemical properties of chiral molecules will be discussed. Attendees will learn:

To recognize racemic mixture
Techniques to measure percent purity of enantiomers
To understand capillary electrophoresis techniques in chiral separation
HPLC instrumentation
To separate racemic mixture by a suitable chiral column
Additionally, the economics and applications of optically active chiral pharmaceutical will be addressed.

Course Description

This course addresses analytical methods commonly employed to investigate racemic mixture. Issues to be discussed include:

Chemistry of chiral molecules
Methods to recognize chiral compounds and enantiomers
Capillary electrophoresis technique in the separation of chiral compounds
Fundamental practices and principles of HPLC
Choice of chiral column and the development of HPLC methods
The importance of mobile phase in the separation of chiral compounds by HPLC
The economics and applications of optically active chiral pharmaceuticals

COURSE AGENDA

DAY ONE
What is Chirality?
-Types and characteristics
-Factors affecting chirality

Properties of Chiral Compounds
-Physical
-Chemical

Measurement and Analysis Not Involving Separation
-Polarimetry
-NMR spectroscopy
-Enzymatic

Capillary Electrophoresis Technique
-Theory and operation
-Methods development

DAY TWO
Analysis and Separation of Chiral Compounds By Chromatography
-GC
-HPLC

Fundamental Practices and Principles of HPLC
-Instrumentation
-Types of chiral columns
-Normal and reversed-phase
-The importance of mobile phase selection
-Analytical methods development

Pharmaceutical Applications
-Examples of current FDA-approved racemic drugs
-Biological activity of single isomer and racemic mixture
-Economic issues

Questions and Answers