Advanced Bacterial Genetics

The Advanced Bacterial Genetics course presents logic and methods used in the genetic dissection of complex biological processes in diverse bacteria. Laboratory methods include: classical mutagenesis using transposons, mutator strains, and chemical and physical mutagens; detection and quantitation of gene expression changes using various reporter genes and real-time PCR; the mapping of mutations using genetic and physical techniques; modern approaches to the generation and analysis of targeted gene disruptions and fusions using PCR and cloning methods; epitope insertion mutagenesis; site-directed mutagenesis; and fluorescence microscopy. Key components of the course will be the use of sophisticated genetic methods in the analysis of model bacteria (including E. coli, Salmonella, Bacillus subtilis, and Myxococcus xanthus), and the use of the wealth of new genomic sequence information to motivate these methods.

Invited lecturers will present various genetic approaches to study bacterial mechanisms of metabolism, development, and pathogenesis.

http://meetings.cshl.edu/course/Default.aspx